kato iii human gastric cancer cell lines  (ATCC)

Journal: International Journal of Molecular Sciences

Article Title: Transcriptomic Profiling Reveals AKR1C1 and AKR1C3 Mediate Cisplatin Resistance in Signet Ring Cell Gastric Carcinoma via Autophagic Cell Death

doi: 10.3390/ijms222212512

Figure Lengend Snippet: Response of parental drug-sensitive KATOIII and drug-resistant KATO/DDP cells to cisplatin cytotoxicity. KATOIII and KATO/DDP cells were treated with different concentrations of cisplatin (0–100 µM) for 48 h, and cell viability was assessed by trypan blue cell exclusion assay. ( A ) Cell viability of KATOIII and KATO/DDP. ( B ) IC 50 value of cisplatin in KATOIII and KATO/DDP cell line. The data are presented as means; * represents p < 0.05 when compared to control.

Article Snippet: The KATOIII human gastric cancer cell line was purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Exclusion Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Transcriptomic Profiling Reveals AKR1C1 and AKR1C3 Mediate Cisplatin Resistance in Signet Ring Cell Gastric Carcinoma via Autophagic Cell Death

doi: 10.3390/ijms222212512

Figure Lengend Snippet: Identification of differentially expressed genes between KATOIII and KATO/DDP cells. ( A ) Volcano diagram of differentially expressed genes between KATO/DDP and KATOIII cell lines, showing 174 upregulated and 345 downregulated genes. ( B ) To validate the differentially expressed genes from RNA sequencing, the expression level of five upregulated and downregulated genes were examined by using qPCR.

Article Snippet: The KATOIII human gastric cancer cell line was purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: RNA Sequencing, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Transcriptomic Profiling Reveals AKR1C1 and AKR1C3 Mediate Cisplatin Resistance in Signet Ring Cell Gastric Carcinoma via Autophagic Cell Death

doi: 10.3390/ijms222212512

Figure Lengend Snippet: Determination of cisplatin resistance related genes in SRCGC. ( A ) Fold-change difference in expression of known drug-resistance-related genes between KATO/DDP and KATOIII gastric cancer cell lines by qPCR. ( B ) Fold-change difference in expression of aldoketoreductase family between KATO/DDP and KATOIII gastric cancer cell line by qPCR. ( C ) Relative expression of aldoketoreductase family in KATO/DDP cell line. ( D ) KATO/DDP and KATOIII gastric cancer cell lines were treated with different concentration of cisplatin (0–10 µM) for 24 h. The expression levels of AKR1C1 and AKR1C3 were detected by Western Blot analysis. ( E ) Quantitative representation of AKR1C1 protein expression by Western blot. ( F ) Quantitative representation of AKR1C3 protein expression by Western blot analysis. Data are presented in mean ± SD; ** represents p < 0.01.

Article Snippet: The KATOIII human gastric cancer cell line was purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Concentration Assay, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Transcriptomic Profiling Reveals AKR1C1 and AKR1C3 Mediate Cisplatin Resistance in Signet Ring Cell Gastric Carcinoma via Autophagic Cell Death

doi: 10.3390/ijms222212512

Figure Lengend Snippet: Role of intracellular ROS in AKR1C1 and 1C3 mediating chemo-resistance in signet ring gastric cancer cells. ( A ) Intracellular ROS level in KATOIII and KATO/DDP cell lines after 1 h treated with cisplatin was detected by DCF-DA ROS assay. ( B ) KATOIII cells were treated with cisplatin with or without NAC for 48 h at 37 °C. Cell viability was assessed by using trypan blue cell exclusion assay. ( C ) Intracellular ROS level in KATO/DDP cells after treatment with cisplatin and ASP9521 or 5PBSA. KATO/DDP cells were pretreated with ASP9521 or 5PBSA at 10 µM for 3 h and added with cisplatin at 25 µM. Intracellular ROS was measured by DCF-DA ROS assay after 1 h of cisplatin treatment. ( D ) Cell viability of KATO/DDP cells treated with cisplatin and ASP9521 or 5PBSA with or without NAC. Data are presented as mean ± SD; * represents p < 0.05 and ** represents p < 0.01.

Article Snippet: The KATOIII human gastric cancer cell line was purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: ROS Assay, Exclusion Assay